Automated Liquid Handling Applications

PCR Reaction Setup and Ampure XR Application

The Biomek 4000 Laboratory Automation Workstation has been used to demonstrate highly reproducible real-time amplification (CV<2%) after automated reaction setup.
In addition, the Agencourt AMPure XP reagent system has been automated on the Biomek 4000 Workstation to provide rapid amplicon purification (two 96-well plates in less than 90 minutes) with yield and purity equivalent to manual purification. Both procedures were demonstrated to be free of cross-contamination.. 

Flexible ELISA automation with the Biomek i5 Workstation

• Automated ELISA assay workflow
• Optimized liquid transfers eliminate the need for a plate washer
• High-capacity deck accommodates fresh tip usage  

Enzyme-linked immunosorbent assays (ELISAs) are a common analytical tool for detecting the presence of a protein or other  molecules of interest. While the workflow is broadly conserved (Figure 1), numerous reagent addition and wash steps make this a labor-intensive endeavor. Automation of these steps not only relieves this labor burden but can lead to improvements in results consistency. Here we demonstrate the automation of an IL-ELISA (R&D Systems) on the Biomek i5 Automated Workstation.