Join us for a day of discovery and innovation as we explore the fascinating world of nanoparticle and virus analysis. Our distinguished speakers will present groundbreaking research and share their insights on the latest techniques in Analytical Science.


Symposium details:


Date: October 12th, 2023

Time: 9:30 a.m. – 2:30 p.m.

Location: CIC, 1 Broadway, Cambridge, MA 02142



Agenda:


October 12th - Morning Session

9:30 a.m.  – 9:45 a.m.  Coffee/sign in/networking
9:45 a.m. - 9:50  a.m. Thank you and Agenda/Beckman Coulter Life Sciecens intros
9:55 a.m. - 10:25  a.m. Determination of mRNA copy number in degradable lipid nanoparticles via density contrast analytical ultracentrifugation

Dr. Alexander Bepperling
10:25 a.m. - 10:35  a.m. Q&A
10:40 a.m. - 11:10  a.m. Rapid high-resolution size distribution analysis for adeno-associated virus using high speed SV-AUC

Dr. Nicholas Larson
11:10 a.m. - 11:20  a.m. Q&A
11:25 a.m. - 11:55  a.m. Characterization of lipid nanoparticles via density matching AUC

Dr. Amy Henrickson
11:55 a.m. - 12:05  p.m. Q&A
12:05 p.m. - 12:50 p.m. Lunch

Register now for free!

October 12th - Afternoon Session

12:55 p.m. - 1:25 p.m. Orthogonal AUC approaches for high-resolution characterization of adeno-associated viruses

Dr. Akash Bhattacharya
1:25 p.m. - 1:35 p.m. Q&A
1:40 p.m. - 2:10 p.m. SV-AUC is a Vital First-Principles Technique in Gene Therapy for Bridging Between Differently Weighted Techniques and for Resolving Interpretation Errors

Dr. Ron Toth
2:10 p.m. - 2:20 p.m. Q&A
2:25 p.m. - 2:30 p.m. Thank You and Closeout


Speakers


Dr. Ronald Toth:


Ron received his Ph.D. in physical chemistry at the University of New Hampshire in the lab of Dr. Thomas Laue - his thesis research focused on using Analytical Ultracentrifugation to investigate the fundamental principles underlying molecular interactions such as aggregation and self-association. Subsequently, Ron served as a post-doc in the labs of Russ Middaugh and David Volkin focusing on biophysical characterization, formulation screening, and Hydrogen Exchange Mass Spectrometry. Ron's first position in industry was as a biophysical scientist for Drug Product Development at GSK Vaccines - Here Ron led a Product Understanding Taskforce, a High-Throughput Automation Working Group, and also developed applications in the R language to help colleagues design experiments and analyze data. At Sanofi Ron has joined the Genomic Medicine Unit in the Characterization group - he leads a small team supporting internal groups with advanced characterization packages and mentoring for biophysical methods!


Dr. Alexander Bepperling:


Alexander Bepperling graduated in the group of Prof. Rainer Rudolph (University of Halle) as biochemist and received his Ph.D. in biotechnology at the chair of Prof. Johannes Buchner (TU Munich). He joined Novartis in 2011 as a scientist and is currently a Lab Head in the department of Biophysical Characterization. He is also leading the Novartis “center of excellence” for analytical ultracentrifugation and the Novartis analytical network for higher order structure analysis of proteins.

Dr. Amy Henrickson:


Amy Henrickson is the manager of the Canadian Center for Hydrodynamics at the University of Lethbridge. She successfully defended her Ph.D. in the laboratory of Prof. Borries Demeler and is an expert in analytical ultracentrifugation (AUC) and a Canada 150 Chair in Biophysics. She is the recipient of multiple scholarships and grants, and her research has been recognized with the prestigious NSERC CGS-D and CGS-M award. Her research targets the development of AUC methods, with a special focus on the characterization of gene therapy products. She is known for her groundbreaking hydrodynamic developments that apply multiwavelength AUC, D2O density matching, and analytical buoyant density equilibrium (ABDE) methods to quantify cargo loading for viral vectors, including AAVs, and LNPs. Her contributions also include the application of these innovative strategies to systems outside of gene therapy drugs, with an emphasis on the characterization of complex biopolymer interactions, including protein-nucleic acid and RNA-RNA interactions, in a large range of biomedical research projects.

Dr. Akash Bhattacharya:


Akash Bhattacharya graduated from Presidency College, India with a major in Physics and went on to a Master’s in Physics at the Indian Institute of Science where he worked on Quantum Computing. He then moved for doctoral studies in Biophysics to the University of Michigan, Ann Arbor where he joined the lab of Prof. Erik Zuiderweg and worked on NMR spectroscopy methods development in the context of the Structural Biology of Chaperone Proteins. After Michigan, he worked briefly at Rutgers and then eventually moved to the Dept. of Biochemistry and Structural Biology at the University of Texas Health at San Antonio. Here, he worked with Prof. Dmitri Ivanov and Prof. Borries Demeler on the enzymology of HIV infection and restriction by mammalian proteins. He also worked on projects related to oncology (DNA damage repair) and neuroscience (voltage gated ion channels), using a wide variety of techniques ranging from X-ray crystallography, NMR spectroscopy, fluorescence spectroscopy, molecular dynamics and analytical ultracentrifugation (AUC). He collaborated with Prof. Demeler to extend AUC methods to novel enzymatic systems resulting in publications in PNAS, Cell Reports, Nature Scientific Reports, etc. Akash joined Beckman Coulter Life Sciences in Oct 2018. He is based in the Colorado R&D centre and works on developing new AUC applications. His research interests include extending AUC methodology to new therapeutic areas such as AAV capsids (gene therapy), liposomal drug carriers and others.

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