Clumpy, aggregated or difficult‑to‑dissociate cell lines continue to be a major challenge in automated cell analysis.

Two of our current application notes provide practical, data‑backed strategies to optimize cell counting accuracy on the Vi‑CELL BLU Cell Viability Analyzer.

What you’ll learn:

1. How to optimize cell type settings for clumpy cells

	Adjusting Decluster Degree improves recognition of cells within aggregates
	When to apply additional Aspiration & Mixing Cycles to reduce clumping
	How to interpret Cluster Count as an indicator of culture health

2. How to improve sample prep for highly aggregated cultures

	Comparison of dissociation media (Trypsin, Trypsin‑EDTA, TrypLE) and their impact on cluster reduction
	The role of gentle trituration for improving counting accuracy
	How a simple side‑sample technique can improve dispersion without changing your passage workflow

Why this matters in your lab

	Increases accuracy of viability/density measurements
	Prevents culture failures by identifying harmful clustering early
	Ensures reliable downstream results
	Maximizes performance of your Vi‑CELL BLU analyzer